Journal: Stem Cell Research & Therapy

Article Title: Exosomes derived from human umbilical cord mesenchymal stem cells inhibit vein graft intimal hyperplasia and accelerate reendothelialization by enhancing endothelial function

doi: 10.1186/s13287-020-01639-1

Figure Lengend Snippet: Morphology and characterization of hucMSCs and hucMSC-exosomes. a The fibroblast-like morphology of hucMSCs shown in light microscopy images (× 40). b Surface markers of hucMSCs analysed by flow cytometry. hucMSCs were positive for CD29, CD44, and CD90 and were negative for CD34 and CD45. c MSCs displayed the ability of adipogenic differentiation (× 40). d MSCs displayed the ability of chondrogenic differentiation (× 40). e Morphology of exosomes under transmission electron microscopy. Scale bar, 100 nm. f The size distribution of exosomes measured by Image-Pro Plus software. g Western blotting analyses of the exosome surface markers (CD9, CD81, and CD63)

Article Snippet: Blots were incubated with the appropriate primary antibodies, including CD9 (1:1000, Proteintech), CD63 (1:1000, Proteintech), CD81 (1:1000, BOSTER, Wuhan, China), inducible nitric oxide synthase (iNOS,1: 1000, Abcam), endothelial nitric oxide synthase (eNOS, 1:1000, Abcam), VEGF-A (1: 1000, Abcam), CD31 (1:1000, Abcam), AKT (1:1000,Cell Signaling, Boston, USA), p-AKT(,1:1000, Cell Signaling), ERK1/2 (1:1000, Cell Signaling), p-ERK1/2 (1:1000, Cell Signaling), and GAPDH (1:2000, Proteintech).

Techniques: Light Microscopy, Flow Cytometry, Transmission Assay, Electron Microscopy, Software, Western Blot

Journal: eLife

Article Title: The PMA phorbol ester tumor promoter increases canonical Wnt signaling via macropinocytosis

doi: 10.7554/eLife.89141

Figure Lengend Snippet:

Article Snippet: Recombinant DNA reagent , CD63-RFP ( Homo sapiens ) (plasmid) , Addgene , RRID: Addgene_62964 , RFP tag.

Techniques: Recombinant, Dominant Negative Mutation, Plasmid Preparation, Membrane, Luciferase, Expressing, Reporter Assay, Software, Imaging, Microinjection, Inverted Microscopy

Journal: Journal of Bone Oncology

Article Title: Expression profiling of DNA methyl transferase I (DNMT1) and efficacy of a DNA-hypomethylating agent (decitabine) in combination with chemotherapy in osteosarcoma

doi: 10.1016/j.jbo.2020.100321

Figure Lengend Snippet: Association of DNMT1 expression levels and sensitivity to decitabine. (A) Expression levels of DNMT1 in osteoblast (hFOB 1.19) and osteosarcoma cell lines (MNNG/HOS, 143B, U2OS, SaOS2, and MG63). (B) Percentage of cell viability of osteosarcoma cell lines measured using MTT assay after treatment with decitabine at indicated concentrations for 72 h.

Article Snippet: Osteosarcoma and osteoblastic cell lines used in this study include 143B (CRL‐8303), MG63 (CRL-1427), SaOS2 (HTB-85), and normal human osteoblast cell line, hFOB1.19 (CRL‐11372) purchased from ATCC (Manassas, VA, USA).

Techniques: Expressing, MTT Assay

Journal: Journal of Bone Oncology

Article Title: Expression profiling of DNA methyl transferase I (DNMT1) and efficacy of a DNA-hypomethylating agent (decitabine) in combination with chemotherapy in osteosarcoma

doi: 10.1016/j.jbo.2020.100321

Figure Lengend Snippet: Percentage inhibition of decitabine in combination with a chemotherapeutic drug (cisplatin or doxorubicin) at indicated concentrations for 72 h in 143B, MNNG/HOS, and MG63 cells.

Article Snippet: Osteosarcoma and osteoblastic cell lines used in this study include 143B (CRL‐8303), MG63 (CRL-1427), SaOS2 (HTB-85), and normal human osteoblast cell line, hFOB1.19 (CRL‐11372) purchased from ATCC (Manassas, VA, USA).

Techniques: Inhibition

Journal: Journal of Bone Oncology

Article Title: Expression profiling of DNA methyl transferase I (DNMT1) and efficacy of a DNA-hypomethylating agent (decitabine) in combination with chemotherapy in osteosarcoma

doi: 10.1016/j.jbo.2020.100321

Figure Lengend Snippet: Synergistic effects of combinations of decitabine and chemotherapeutic drugs in 143B, MNNG/HOS and MG63 cells. Synergy scores were calculated using Synergyfinder software. ZIP Synergy scores > 0 indicate synergism (red regions) and scores < 0 indicate antagonism (green regions). Values in the white boxes represent the average synergy score for the region of highest synergy. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Osteosarcoma and osteoblastic cell lines used in this study include 143B (CRL‐8303), MG63 (CRL-1427), SaOS2 (HTB-85), and normal human osteoblast cell line, hFOB1.19 (CRL‐11372) purchased from ATCC (Manassas, VA, USA).

Techniques: Software